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bioRxiv · 10.1101/572958

Looking for Therapeutic Antibodies in Next Generation Sequencing Repositories

Krawczyk, K.·Kovaltsuk, A.·Raybould, M.·Deane, C. M.
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Abstract

Recently it has become possible to query the great diversity of natural antibody repertoires using Next Generation Sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare Clinical Stage Therapeutic antibodies to the [~]1b sequences from 60 independent sequencing studies in the Observed Antibody Space Database. Of the 242 post Phase I antibodies, we find 16 with sequence identity matches of 95% or better for both heavy and light chains. There are also 54 perfect matches to therapeutic CDR-H3 regions in the NGS outputs, suggesting a nontrivial amount of convergence between naturally observed sequences and those developed artificially. This has potential implications for both the discovery of antibody therapeutics and the legal protection of commercial antibodies.

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Krawczyk, K., Kovaltsuk, A., Raybould, M., Deane, C. M.. 2019-03-09. Looking for Therapeutic Antibodies in Next Generation Sequencing Repositories. https://doi.org/10.1101/572958

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