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bioRxiv · 10.1101/572826

A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors

Lapin, D.·Kovacova, V.·Sun, X.·Dongus, J. A.·Bhandari, D. D.·von Born, P.·Bautor, J.·Guarneri, N.·Stuttmann, J.·Beyer, A.·Parker, J. E.
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Abstract

Plant intracellular nucleotide-binding/leucine-rich repeat (NLR) immune receptors are activated by pathogen effectors to trigger host defenses and cell death. Toll-Interleukin1-receptor (TIR)-domain NLRs (TNLs) converge on the Enhanced Disease Susceptibility1 (EDS1) family of lipase-like proteins for all resistance outputs. In Arabidopsis TNL immunity, AtEDS1 heterodimers with Phytoalexin Deficient4 (AtPAD4) transcriptionally boost basal defense pathways. AtEDS1 uses the same surface to interact with PAD4-related Senescence-Associated Gene101 (AtSAG101), but the role of AtEDS1-AtSAG101 heterodimers was unclear. We show that AtEDS1-AtSAG101 function together with AtNRG1 coiled-coil domain helper NLRs as a coevolved TNL cell death signaling module. AtEDS1-AtSAG101-AtNRG1 cell death activity is transferable to the solanaceous species, Nicotiana benthamiana, and cannot be substituted by AtEDS1-AtPAD4 with AtNRG1 or AtEDS1-AtSAG101 with endogenous NbNRG1. Analysis of EDS1-family evolutionary rate variation and heterodimer structure-guided phenotyping of AtEDS1 variants or AtPAD4-AtSAG101 chimeras identify closely aligned -helical coil surfaces in the AtEDS1-AtSAG101 partner C-terminal domains that are necessary for TNL cell death signaling. Our data suggest that TNL-triggered cell death and pathogen growth restriction are determined by distinctive features of EDS1-SAG101 and EDS1-PAD4 complexes and that these signaling machineries coevolved with further components within plant species or clades to regulate downstream pathways in TNL immunity.

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Lapin, D., Kovacova, V., Sun, X., Dongus, J. A., Bhandari, D. D., von Born, P., Bautor, J., Guarneri, N., Stuttmann, J., Beyer, A., Parker, J. E.. 2019-03-11. A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors. https://doi.org/10.1101/572826

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