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bioRxiv · 10.1101/568915

CUT&Tag for efficient epigenomic profiling of small samples and single cells

Kaya-Okur, H. S.·Wu, S. J.·Codomo, C. A.·Pledger, E. S.·Bryson, T. D.·Henikoff, J. G.·Ahmad, K.·Henikoff, S.
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Abstract

Many chromatin features play critical roles in regulating gene expression. A complete understanding of gene regulation will require the mapping of specific chromatin features in small samples of cells at high resolution. Here we describe Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components. In CUT&Tag, a chromatin protein is bound in situ by a specific antibody, which then tethers a protein A-Tn5 transposase fusion protein. Activation of the transposase efficiently generates fragment libraries with high resolution and exceptionally low background. All steps from live cells to sequencing-ready libraries can be performed in a single tube on the benchtop or a microwell in a high-throughput pipeline, and the entire procedure can be performed in one day. We demonstrate the utility of CUT&Tag by profiling histone modifications, RNA Polymerase II and transcription factors on low cell numbers and single cells.

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Kaya-Okur, H. S., Wu, S. J., Codomo, C. A., Pledger, E. S., Bryson, T. D., Henikoff, J. G., Ahmad, K., Henikoff, S.. 2019-03-06. CUT&Tag for efficient epigenomic profiling of small samples and single cells. https://doi.org/10.1101/568915

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