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bioRxiv · 10.1101/566315

Digital removal of autofluorescence from microscopy images

Baharlou, H.·Canete, N. P.·Bertram, K. M.·Sandgren, K. J.·Cunningham, A. L.·Harman, A. N.·Patrick, E.
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Abstract

Autofluorescence is a long-standing problem that has hindered fluorescence microscopy image analysis. To address this, we have developed a method that identifies and removes autofluorescent signals from multi-channel images post acquisition. We demonstrate the broad utility of this algorithm in accurately assessing protein expression in situ through the removal of interfering autofluorescent signals. Availability and implementationhttps://ellispatrick.github.io/AFremover Contactellis.patrick@sydney.edu.au Supplementary informationSupplementary Figs. 1-13

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Baharlou, H., Canete, N. P., Bertram, K. M., Sandgren, K. J., Cunningham, A. L., Harman, A. N., Patrick, E.. 2019-03-03. Digital removal of autofluorescence from microscopy images. https://doi.org/10.1101/566315

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