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bioRxiv · 10.1101/566109

Sensitive determination of proteolytic proteoforms in limited microscale proteome samples

Weng, S. S. H.·Demir, F.·Ergin, E. K.·Dirnberger, S.·Uzozie, A.·Tuscher, D.·Nierves, L.·Tsui, J.·Huesgen, P. F.·Lange, P. F.
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Abstract

Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. Current terminome identification approaches are limited to whole organs or expandable cultured cells. We present a robust, sensitive, scalable and automatable method for system-wide identification of thousands of N-termini from minute samples. Identification of distinct N- terminal profiles in sorted immune cells, subcellular compartments, clinical biopsies, plasma from pediatric cancer patients, and protease substrates in Arabidopsis seedlings demonstrate broad applicability.

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Weng, S. S. H., Demir, F., Ergin, E. K., Dirnberger, S., Uzozie, A., Tuscher, D., Nierves, L., Tsui, J., Huesgen, P. F., Lange, P. F.. 2019-03-03. Sensitive determination of proteolytic proteoforms in limited microscale proteome samples. https://doi.org/10.1101/566109

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