Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons

Abstract

The integration of synaptic inputs onto dendrites provides the basis for computation within individual neurons. Whereas recent studies have begun to outline the spatial organization of synaptic inputs on individual neurons, the underlying principles related to the specific neural functions is not well known. Here we performed two-photon dendritic imaging with genetically-encoded glutamate sensor in awake monkeys, and successfully mapped the excitatory synaptic inputs on dendrites of individual V1 neurons with high spatial and temporal resolution. We found that although synaptic inputs on dendrites were functionally clustered by feature, they were highly scattered in multidimensional feature space, providing a potential substrate of local feature integration on dendritic branches. We also found that nearly all individual neurons received both abundant orientation-selective and color-selective inputs. Furthermore, we found apical dendrites received more diverse inputs than basal dendrites, with larger receptive fields, and relatively longer response latencies, suggesting a specific apical role in integrating feedback in visual information processing.