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bioRxiv · 10.1101/557637

The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter

Abstract

Bacterial growth and division require regulated synthesis of the macromolecules used to expand and replicate components of the cell. Transcription of housekeeping genes required for metabolic homeostasis and cell proliferation is guided by the sigma factor {sigma}70. The conserved CarD-like transcriptional regulator, CdnL, associates with promoter regions where {sigma}70 localizes and stabilizes the open promoter complex. However, the contributions of CdnL to metabolic homeostasis and bacterial physiology are not well understood. Here, we show that Caulobacter crescentus cells lacking CdnL have severe morphological and growth defects. Specifically,{Delta} cdnL cells grow slowly in both rich and defined media, and are wider, more curved, and have shorter stalks than WT cells. These defects arise from transcriptional downregulation of most major classes of biosynthetic genes. Notably, we find that{Delta} cdnL cells are severely limited in glutamate synthesis, rendering them auxotrophic for that amino acid. Moreover,{Delta} cdnL cells produce low amounts of the cell wall precursor lipid II, and{Delta} cdnL is synthetic lethal with other genetic perturbations that limit lipid II production.{Delta} cdnL cells also have aberrant localization of MreB and CtpS, cytoskeletal proteins required for maintaining proper cell width and curvature. Interestingly, the localization of CtpS is dependent on availability of CTP, which is predicted to be low in{Delta} cdnL cells. Our findings implicate CdnL as a global regulator of genes required for metabolic homeostasis that impacts morphogenesis through availability of lipid II and through metabolite-mediated changes in localization of cytoskeletal regulators of cell shape.

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BibTeXRIS

Woldemeskel, S. A., Alvarez, L., Daitch, A. K., Zeinert, R., Bhargava, A., Gonzalez, D., Collier, J., Chien, P., Cava, F., Goley, E.. 2019-02-22. The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter. https://doi.org/10.1101/557637

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