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bioRxiv · 10.1101/556209

How prolonged expression of Hunchback, a temporal transcription factor, re-wires locomotor circuits

Heckscher, E.·Meng, J. L.·Marshall, Z. D.·Lobb-Rabe, M.
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Abstract

AbstractIn many CNS regions, neuronal birth timing is associated with circuit membership. In Drosophila larvae, we show U motor neurons are a temporal cohort--a set of non-identical, contiguously-born neurons from a single neuronal stem cell that contribute to the same circuit. We prolong expression of a temporal transcription factor, Hunchback, to increase the number of U motor neurons with early-born molecular identities. On the circuit level, this expands and re-wires the U motor neuron temporal cohort. On the cell biological level, we find novel roles for Hunchback in motor neuron target selection, neuromuscular synapse formation, dendrite morphogenesis, and behavior. These data provide insight into the relationship between stem cell and circuit, show that Hunchback is a potent regulator of circuit assembly, and suggest that temporal transcription factors are molecules that could be altered during evolution or biomedical intervention for the generation of novel circuits.

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Heckscher, E., Meng, J. L., Marshall, Z. D., Lobb-Rabe, M.. 2019-02-20. How prolonged expression of Hunchback, a temporal transcription factor, re-wires locomotor circuits. https://doi.org/10.1101/556209

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