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bioRxiv · 10.1101/554337

Localization microscopy at doubled precision with patterned illumination

Cnossen, J.·Hinsdale, T.·Thorsen, R.·Schueder, F.·Jungmann, R.·Smith, C. S.·Rieger, B.·Stallinga, S.
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Abstract

MINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field-of-view and a lack of practical parallelism. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures.

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Cnossen, J., Hinsdale, T., Thorsen, R., Schueder, F., Jungmann, R., Smith, C. S., Rieger, B., Stallinga, S.. 2019-02-20. Localization microscopy at doubled precision with patterned illumination. https://doi.org/10.1101/554337

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