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bioRxiv · 10.1101/545566

Recombinant production and purification of the human protein Tau

Ferrari, L.·Rudiger, S. G. D.
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Abstract

Tau protein is a microtubule-stabilizing protein whose aggregation is linked to Alzheimers Disease and other forms of dementia. Tau biology is at the heart of cytoskeletal dynamics and neurodegenerative mechanisms, making it a crucial protein to study. Tau purification, however, is challenging as Tau is disordered, which makes it difficult to produce in recombinant system and is degradation-prone. It is thus challenging to obtain pure and stable preparations of Tau. Here, we present a fast and robust protocol to purify Tau recombinantly in Escherichia coli. Our protocol allows purifyig Tau either tag-less free or FLAG-tagged at its N-terminus. By exploiting a cleavable affinity tag and two anion exchange columns, we obtained Tau is of high purity, stable and suitable for in vitro studies, including aggregation experiments that resemble neurodegenerative processes.

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Ferrari, L., Rudiger, S. G. D.. 2019-02-20. Recombinant production and purification of the human protein Tau. https://doi.org/10.1101/545566

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