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bioRxiv · 10.1101/540104

Transcriptional kinetics of X-chromosome upregulation

Larsson, A. J. M.·Coucoravas, C.·Sandberg, R.·Reinius, B.
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Abstract

Ohnos hypothesis postulates that X-chromosome upregulation rectifies X-dose imbalance relative to autosomal genes, present in two active copies per cell. Here we dissected X-upregulation into kinetics of transcription, inferred from allele-specific single-cell RNA-sequencing (scRNAseq) data from somatic mouse cells. We confirmed increased X-chromosome expression, and remarkably found that the X-chromosome achieved upregulation by elevated burst frequencies. This provides mechanistic insights into X-chromosome upregulation.

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Larsson, A. J. M., Coucoravas, C., Sandberg, R., Reinius, B.. 2019-02-05. Transcriptional kinetics of X-chromosome upregulation. https://doi.org/10.1101/540104

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