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bioRxiv · 10.1101/540039

Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis

Abstract

Changes in metabolism can alter the cellular milieu; can this also change intracellular proteostasis? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change proteostasis, arguably, it should also alter mutational buffering. Building on this, we find that altered cellular metabolic states in E. coli buffer distinct mutations. Buffered-mutants had folding problems in vivo and were differently chaperoned in different metabolic states. Notably, this assistance was dependent upon the metabolites and not on the increase in canonical chaperone machineries. Additionally, we were able to reconstitute the folding assistance afforded by metabolites in vitro and propose that changes in metabolite concentrations have the potential to alter proteostasis. Collectively, we unravel that the metabolite pools are bona fide members of proteostasis and aid in mutational buffering. Given the plasticity in cellular metabolism, we posit that metabolic alterations may play an important role in the positive or negative regulation of proteostasis.

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BibTeXRIS

Verma, K., Saxena, K., Donaka, R., Chaphalkar, A., Rai, M. K., Shukla, A., Zaidi, Z., Dandage, R., Shanmugam, D., Chakraborty, K.. 2019-02-06. Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis. https://doi.org/10.1101/540039

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