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bioRxiv · 10.1101/537928

retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation

Reinert, J. K.·Sonntag, I.·Sonntag, H.·Sprengel, R.·Pelzer, P.·Lessle, S.·Kaiser, M.·Kuner, T.
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Abstract

Identifying and manipulating synaptically connected neurons across brain regions remains a core challenge in understanding complex nervous systems. RetroLEAP is a novel approach for retrograde trans-synaptic Labelling, Expression And Perturbation. GFP-dependent recombinase (Flp-DOG) detects trans-synaptic transfer of GFP-tetanus toxin heavy chain fusion protein (GFP-TTC) and activates expression of any gene of interest in synaptically connected cells. RetroLEAP overcomes existing limitations, is non-toxic, highly flexible, efficient, sensitive and easy to implement.

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Reinert, J. K., Sonntag, I., Sonntag, H., Sprengel, R., Pelzer, P., Lessle, S., Kaiser, M., Kuner, T.. 2019-02-01. retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation. https://doi.org/10.1101/537928

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