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bioRxiv · 10.1101/535039

Septin 9-containging filaments and Golgi assembly depend on two polybasic domains

Abstract

Septins are GTP-binding proteins involved in several membrane remodeling mechanisms. They associate with membranes, presumably by using a polybasic domain (PB1) that interacts with phosphoinositides (PIs). Membrane-bound septins assemble into microscopic structures that regulate membrane shape. How septins exactly interact with PIs, assemble, and shape membranes is weakly understood. Here, we found that septin 9 has a second polybasic domain (PB2) conserved in the human septin family. Similarly to PB1, PB2 binds specifically to PIs, and both domains are critical for septin filament formation. However, septin 9 membrane association does not depend on these PB domains but on putative PB-adjacent amphipathic helices. The presence of the PB domains guarantees the protein enrichment to PI-contained membranes, which is critical for PI-enriched organelles. In particular, we found that septin 9 PB domains control the assembly and functionality of the Golgi apparatus. Our findings bring novel insights into the role of septins in organelle morphology. HighlightsO_LITwo polybasic domains mediate septin 9 interaction with PIs C_LIO_LIHuman septins have amphipathic helices suitable for binding membrane C_LIO_LISeptin 9 polybasic domains mediate septin high order structure formation C_LIO_LIMutation or depletion of septin polybasic domains induce Golgi fragmentation C_LI

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BibTeXRIS

Omrane, M., Camara, A. S., Taveneau, C., Benzoubir, N., Tubiana, T., Yua, J., Guerois, R., Samuel, D., Goud, B., Pous, C., Bressanelli, S., Garratt, R. C., Thiam, A. R., Gassama-Diagne, A.. 2019-01-30. Septin 9-containging filaments and Golgi assembly depend on two polybasic domains. https://doi.org/10.1101/535039

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