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bioRxiv · 10.1101/533356

A real-time multiplexed microbial growth intervalometer for capturing high resolution growth curves

Vuono, D.·Lipp, B.·Staub, C.·Loney, E.·Grzymski, J.
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Abstract

Batch cultures are a low maintenance and routine culturing method used in anaerobic microbiology. Automated tools that measure growth curves from anaerobic microorganisms grown in traditional laboratory glassware, such as Balch-type tubes, are not commercially available. Here we present a new MicrobiAl Growth Intervalometer (MAGI) that captures microbial growth curves through photo conductivity of the medium using a diffused light pattern of specified frequency, rather than photo-attenuation of collimated light used in traditional systems, and is configured with an offset photodetector/emitter to minimize direct impingement of light from the source to improve the resolution of the solutions density. MAGI is operated by software-driven automation and offers investigators a low noise/high gain instrument with capabilities for remote visualization and data acquisition. MAGI is a low maintenance, low cost, and robust platform primarily for anaerobic cultivation and growth monitoring. We demonstrate the utility of this device by first showing that growth rates and generation times in Escherichia coli K-12 are reproducible to previously published results. We then tested MAGI to measure growth curves of an environmental organism, Intrasporangium calvum, under various media compositions. Our results demonstrate that MAGI is a versatile platform to measure growth curves in media under various redox conditions (microaerobic and anaerobic), complex mediums such as Luria-Bertani (LB) broth and minimal media, and for resolving diauxic growth curves when I. calvum is grown on a disaccharide. Lastly, we demonstrate that the device can resolve growth curves for M concentrations of resources that yield low biomass. This research advances the tools available to microbiologist aiming to monitor growth curves in a variety of disciplines, such as environmental microbiology, clinical microbiology, and food sciences.

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Vuono, D., Lipp, B., Staub, C., Loney, E., Grzymski, J.. 2019-01-31. A real-time multiplexed microbial growth intervalometer for capturing high resolution growth curves. https://doi.org/10.1101/533356

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