The impact of coregistration of gradient recalled echo images on quantitative susceptibility and R2* mapping at 7T

Choi, S.; Li, X.; Harrison, D.

doi:10.1101/529891

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bioRxiv · 10.1101/529891

The impact of coregistration of gradient recalled echo images on quantitative susceptibility and R2* mapping at 7T

Choi, S.·Li, X.·Harrison, D.

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Abstract

IntroductionWhile image coregistration is inevitable and quantitative parametric maps, such as R2* and QSM, are increasingly used in multi-parametric studies of the brain, there is a lack of investigations on the reliability of quantitative metrics after coregistration. The purposes of this study were 1) to evaluate the reliability of R2* and QSM at 7T and 2) to assess the statistical agreement in the quantitative metrics obtained by two different coregistration approaches. MethodsWe compared the reliability of R2* and quantitative susceptibility maps obtained from brains of eight healthy participants by two coregistration approaches: 1) transformation of pre-processed quantitative maps and 2) processing quantitative maps after transformation using pixel- and ROI-based analyses. Two-sample Kolmogorov-Smirnov, Mann-Whitney U, Paired T, Intraclass-correlation tests were performed appropriately. ResultsR2* remained invariant regardless of the cogeneration timing. However, magnetic susceptibility was significantly altered when processed in transformed space, whereas it remained invariant in all KS-tests and pixel values were only different in 2 out of 64 U-tests for direct QSM coregistration. Paired t-test revealed that ROI-based group-mean R2* was invariant to both approaches, while group-mean susceptibility was invariant to direct coregistration but differed in one structure processed in transformed space. For all pairs of measurements of R2*, ICCs were excellent. ICCs for magnetic susceptibility were excellent when processed in its native space while the ICCs were lower than 0.9 or poor when processed in transformed space. Further analysis revealed that the choice of interpolation approach affected the resultant QSM. ConclusionsOur study shows that R2* could be safely processed in a transformed space, whereas QSM was less reliable when processed in the transformed space. Hence, caution is advised when using QSM in a multi-parametric study, and it is strongly recommended to process QSM in its native space prior to any coregistration or spatial transformation.

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Choi, S., Li, X., Harrison, D.. 2019-01-24. The impact of coregistration of gradient recalled echo images on quantitative susceptibility and R2* mapping at 7T. https://doi.org/10.1101/529891

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