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bioRxiv · 10.1101/526137

MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

Templier, T.
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Abstract

The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

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Templier, T.. 2019-01-26. MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy. https://doi.org/10.1101/526137

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