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bioRxiv · 10.1101/521237

Meiotic viral attenuation through an ancestral apoptotic pathway

Meneghini, M. D.·Gao, J.·Chau, S.·Chowdhury, F.·Zhou, T.·Hossain, S.·Mcquibban, A.
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Abstract

The programmed release of apoptogenic proteins from mitochondria is a core event of apoptosis, though ancestral roles of this phenomenon are not known. In mammals, one such apoptogenic protein is Endonuclease G (EndoG), a conserved nuclease that fragments the DNA of dying cells. In this work, we show that budding yeast executes meiotically programmed mitochondrial release of an EndoG homologue, Nuc1, during sporulation. In contrast to EndoGs ostensible pro-death function during apoptosis, Nuc1 mitochondrial release attenuates the cytosolic dsRNA mycovirus, Killer, protecting spores from a lethal accumulation of its encoded toxin. Our identification of cell-protective viral attenuation as a target of this rudimentary apoptotic pathway illuminates a primordial role for mitochondrial release of EndoG.\n\nOne Sentence SummaryYeast sporulation induces release of mitochondrial endonuclease G to accomplish viral attenuation.

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Meneghini, M. D., Gao, J., Chau, S., Chowdhury, F., Zhou, T., Hossain, S., Mcquibban, A.. 2019-01-15. Meiotic viral attenuation through an ancestral apoptotic pathway. https://doi.org/10.1101/521237

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