A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes

Imai, R.; Nagai, M.; Sakaguchi, S.; Masuda, T.; Kuroda, M.; Oba, M.; Katayama, Y.; Naoi, Y.; Tsuchiaka, S.; Omatsu, T.; Yamazato, H.; Makino, S.; Mizutani, T.

doi:10.1101/510131

bioRxiv · 10.1101/510131

A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes

Imai, R.·Nagai, M.·Sakaguchi, S.·Masuda, T.·Kuroda, M.·Oba, M.·Katayama, Y.·Naoi, Y.·Tsuchiaka, S.·Omatsu, T.·Yamazato, H.·Makino, S.·Mizutani, T.

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Abstract

Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.

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Imai, R., Nagai, M., Sakaguchi, S., Masuda, T., Kuroda, M., Oba, M., Katayama, Y., Naoi, Y., Tsuchiaka, S., Omatsu, T., Yamazato, H., Makino, S., Mizutani, T.. 2019-01-02. A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes. https://doi.org/10.1101/510131

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