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bioRxiv · 10.1101/504142

Diagnostic high-throughput sequencing of 2,390 patients with bleeding, thrombotic and platelet disorders

Abstract

A targeted high-throughput sequencing (HTS) panel test for clinical diagnostics requires careful consideration of the inclusion of appropriate diagnostic-grade genes, the ability to detect multiple types of genomic variation with high levels of analytic sensitivity and reproducibility, and variant interpretation by a multi-disciplinary team (MDT) in the context of the clinical phenotype. We have sequenced 2,390 index patients using the ThromboGenomics HTS panel test of diagnostic-grade genes known to harbour variants associated with rare bleeding, thrombotic or platelet disorders (BPD). The diagnostic rate was determined by the clinical phenotype, with an overall rate of 50.4% for all thrombotic, coagulation, platelet count and function disorder patients and a rate of 6.2% for patients with unexplained bleeding disorders characterized by normal hemostasis test results. The MDT classified 756 unique variants, including copy number and intronic variants, as Pathogenic, Likely Pathogenic or Variants of Uncertain Significance. Almost half (49.7%) of these variants are novel and 41 unique variants were identified in 7 genes recently found to be implicated in BPD. Inspection of canonical hemostasis pathways identified 29 patients with evidence of oligogenic inheritance. A molecular diagnosis has been reported for 897 index patients providing evidence that introducing a HTS genetic test for BPD patients is meeting an important unmet clinical need.\n\nKey pointsO_LIHigh-throughput sequencing (HTS) test reveals a molecular diagnosis for 38% of 2,390 patients with bleeding, thrombotic and platelet disorders.\nC_LIO_LIThromboGenomics HTS test validates recent gene discoveries and detects copy number and intronic variants.\nC_LI

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BibTeXRIS

Downes, K., Megy, K., Duarte, D., Vries, M., Gebhart, J., Hofer, S., Shamardina, O., Deevil, S. V., Stephens, J., Mapeta, R., Tuna, S., Al Hasso, N., Besser, M. W., Cooper, N., Daugherty, L., Gleadall, N., Greene, D., Haimel, M., Martin, H., Papadia, S., Revel-Vilk, S., Sivapalaratnam, S., Symington, E., Thomas, W., Thys, C., Tolios, A., Penkett, C. J., NIHR BioResource,, Ouwehand, W. H., Abbs, S., Laffan, M. A., Turro, E., Simeoni, I., Mumford, A. D., Henskens, Y. M., Pabinger, I., Gomez, K., Freson, K.. 2018-12-21. Diagnostic high-throughput sequencing of 2,390 patients with bleeding, thrombotic and platelet disorders. https://doi.org/10.1101/504142

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