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bioRxiv · 10.1101/498261

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

Garcia-Diaz, B.·Bachelin, C.·Coulpier, F.·Gerschenfeld, G.·Deboux, C.·Zujovic, V.·Charnay, P.·Topilko, P.·Baron-Van Evercooren, A.
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Abstract

Schwann cells (SC) enter the central nervous system (CNS) in pathophysiological conditions. However, how SC invade the CNS to remyelinate central axons remains undetermined. We studied SC migratory behavior ex vivo and in vivo after exogenous transplantation in the demyelinated spinal cord. Data highlight for the first time that SC migrate preferentially along blood vessel in perivascular ECM, avoiding CNS myelin. We demonstrate in vitro and in vivo that this migration route occurs by virtue of a dual mode of action of Eph/ephrin receptor. Indeed, EphrinB3, enriched in myelin, interacts with SC Eph receptors, to drive SC away from CNS myelin, and triggers their preferential adhesion to ECM components, such as fibronectin via integrin{beta}1 interactions. This complex interplay enhances SC migration along the blood vessel network and together with lesion-induced vascular remodeling facilitates their timely invasion of the lesion site. These novel findings elucidate the mechanism by which SC invade and contribute to spinal cord repair.

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Garcia-Diaz, B., Bachelin, C., Coulpier, F., Gerschenfeld, G., Deboux, C., Zujovic, V., Charnay, P., Topilko, P., Baron-Van Evercooren, A.. 2018-12-16. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. https://doi.org/10.1101/498261

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