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bioRxiv · 10.1101/495242

Disruption of IRE1α through its Kinase Domain Attenuates Multiple Myeloma

Abstract

Multiple myeloma (MM) arises from malignant immunoglobulin-secreting plasma cells and remains an incurable, often lethal disease despite recent therapeutic advances. The unfolded-protein response sensor IRE1 supports protein secretion by deploying a kinase-endoribonuclease module to activate the transcription factor XBP1s. MM cells may coopt the IRE1-XBP1s pathway; however, the validity of IRE1 as a potential MM therapeutic target is controversial. Here we show that genetic disruption of IRE1 or XBP1s, or pharmacologic IRE1 kinase inhibition, attenuated subcutaneous or orthometastatic growth of MM tumors in mice, and augmented efficacy of two well-established frontline antimyeloma agents, bortezomib or lenalidomide. Mechanistically, IRE1 perturbation inhibited expression of key components of the ER-associated degradation machinery, as well as cytokines and chemokines known to promote MM growth. Selective IRE1 kinase inhibition reduced viability of CD138+ plasma cells while sparing CD138- cells from bone marrow of newly diagnosed MM patients or patients whose disease relapsed after 1 - 4 lines of treatment in both US- and EU-based cohorts. IRE1 inhibition preserved survival and glucose-induced insulin secretion by pancreatic microislets. Together, these results establish a strong therapeutic rationale for targeting IRE1 with kinase-based small-molecule inhibitors in MM.\n\nSignificance statementMultiple myeloma (MM) is a lethal malignancy of plasma cells. MM cells have an expanded endoplasmic reticulum (ER) that is constantly under stress due to immunoglobulin hyperproduction. The ER-resident sensor IRE1 mitigates ER stress by expanding the ERs protein-folding capacity while supporting proteasomal degradation of misfolded ER proteins. IRE1 elaborates these functions by deploying its cytoplasmic kinase-RNase module to activate the transcription factor XBP1s. The validity of IRE1 as a potential therapeutic target in MM has been questioned. Using genetic and pharmacologic disruption in vitro and in vivo, we demonstrate that the IRE1-XBP1s pathway plays a critical role in MM growth. We further show that IRE1s kinase domain is an effective and safe potential small-molecule target for MM therapy.

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BibTeXRIS

Harnoss, J. M., Le Thomas, A., Marsters, S. A., Lawrence, D. A., Lu, M., Chen, Y.-C. A., Qing, J., Totpal, K., Kan, D., Segal, E., Ackerly Wallweber, H., Wang, W., Clark, K., Kaufman, S., Beresini, M., Sandoval, W., Lorenzo, M., Wu, J., Ly, J., De Bruyn, T., Heidersbachh, A., Haley, B., Gogineni, A., Weimer, R., Lee, D., Braun, M.-G., Rudolph, J., VanWyngarden, M. J., Sherbenou, D. W., Gomez-Bougie, P., Amiot, M., Acosta-Alvear, D., Walter, P., Ashkenazi, A.. 2018-12-13. Disruption of IRE1α through its Kinase Domain Attenuates Multiple Myeloma. https://doi.org/10.1101/495242

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