Bioinformatic screening of genes present only in well regenerating vertebrates reveals novel FGF and purinergic signaling modulator - c-Answer

Korotkova, D. D.; Lyubetsky, V. A.; Ivanova, A. S.; Rubanov, L. I.; Seliverstov, A. V.; Zverkov, O. A.; Martynova, N. Y.; Nesterenko, A. M.; Tereshina, M. B.; Peshkin, L.; Zaraisky, A. G.

doi:10.1101/494609

bioRxiv · 10.1101/494609

Bioinformatic screening of genes present only in well regenerating vertebrates reveals novel FGF and purinergic signaling modulator - c-Answer

Korotkova, D. D.·Lyubetsky, V. A.·Ivanova, A. S.·Rubanov, L. I.·Seliverstov, A. V.·Zverkov, O. A.·Martynova, N. Y.·Nesterenko, A. M.·Tereshina, M. B.·Peshkin, L.·Zaraisky, A. G.

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Abstract

The genetic basis of higher regenerative capacity of fishes, amphibians and reptiles compared to birds and mammals is still poorly understood. Though it is thought to be a result of restructuring in the regulatory network of a static set of genes, we argued that it could be due to the loss of genes essential for regeneration. In the present work, we formulate a bioinformatic approach to systematic search for the such genes. Having identified them, we further investigated one we dubbed \"c-Answer\", which encodes a membrane protein, regulating the regeneration of body appendages and the telencephalic development through binding to FGFR and P2Y1 receptors and promoting MAPK/ERK and purinergic signaling. The obtained data suggest that elimination of c-Answer in the ancestors of warm-blooded animals conditioned the decreased activity of at least two signaling pathways, which in turn could contribute to changes in mechanisms that regulate the forebrain development and regeneration.

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Korotkova, D. D., Lyubetsky, V. A., Ivanova, A. S., Rubanov, L. I., Seliverstov, A. V., Zverkov, O. A., Martynova, N. Y., Nesterenko, A. M., Tereshina, M. B., Peshkin, L., Zaraisky, A. G.. 2018-12-13. Bioinformatic screening of genes present only in well regenerating vertebrates reveals novel FGF and purinergic signaling modulator - c-Answer. https://doi.org/10.1101/494609

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