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bioRxiv · 10.1101/483016

Early Detection of Neurological Dysfunction Using Blood Cell Transcript Profiles

Moni, M. A.·Rana, H. K.·Islam, M. B.·Ahmed, M. B.·Lio', P.·Hasan, M. A. M.·Huq, F.·Quinn, J.
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Abstract

Identification of genes whose regulation of expression is similar in both brain and blood cells could enable monitoring of significant neurological traits and disorders by analysis of blood samples. We thus employed transcriptional analysis of pathologically affected tissues, using agnostic approaches to identify overlapping gene functions and integrating this transcriptomic information with expression quantitative trait loci (eQTL) data. Here, we estimate the correlation of genetic expression in the top-associated cis-eQTLs of brain tissue and blood cells in Parkinsons (PD). We introduced quantitative frameworks to reveal the complex relationship of various biasing genetic factors in PD, a neurodegenerative disease. We examined gene expression microarray and RNA-Seq datasets from human brain and blood tissues from PD-affected and control individuals. Differentially expressed genes (DEG) were identified for both brain and blood cells to determine common DEG overlaps. Based on neighborhood-based benchmarking and multilayer network topology aproaches we then developed genetic associations of factors with PD. Overlapping DEG sets underwent gene enrichment using pathway analysis and gene ontology methods, which identified candidate common genes and pathways. We identified 12 significantly dysregulated genes shared by brain and blood cells, which were validated using dbGaP (gene SNP-disease linkage) database for gold-standard benchmarking of their significance in disease processes. Ontological and pathway analyses identified significant gene ontology and molecular pathways that indicate PD progression. In sum, we found possible novel links between pathological processes in brain and blood cells by examining cell path-way commonalities, corroborating these associations using well validated datasets. This demonstrates that for brain-related pathologies combining gene expression analysis and blood cell cis-eQTL is a potentially powerful analytical approach. Thus, our methodologies facilitate data-driven approaches that can advance knowledge of disease mechanisms and may enable prediction of neurological dysfunction using blood cell transcript profiling.

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Moni, M. A., Rana, H. K., Islam, M. B., Ahmed, M. B., Lio', P., Hasan, M. A. M., Huq, F., Quinn, J.. 2018-11-29. Early Detection of Neurological Dysfunction Using Blood Cell Transcript Profiles. https://doi.org/10.1101/483016

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