Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy

Slezak, M.; de Vin, F.; Shinmyo, Y.; Batiuk, M. Y.; Rincon, M. Y.; Menacho Pando, C.; Urschitz, J.; Moisyadi, S.; Schnuetgen, F.; Kawasaki, H.; Holt, M. G.

doi:10.1101/481580

bioRxiv · 10.1101/481580

Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy

Slezak, M.·de Vin, F.·Shinmyo, Y.·Batiuk, M. Y.·Rincon, M. Y.·Menacho Pando, C.·Urschitz, J.·Moisyadi, S.·Schnuetgen, F.·Kawasaki, H.·Holt, M. G.

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Abstract

In utero electroporation is increasingly used for manipulation of gene expression in the mammalian central nervous system. Here we created a transposon-based single plasmid system, that allows long-term conditional loss- and gain-of-function experiments across major CNS cell types. The system is widely applicable across species, allowing fast, flexible and cheap cell manipulation in previously non-transgenic species.

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Slezak, M., de Vin, F., Shinmyo, Y., Batiuk, M. Y., Rincon, M. Y., Menacho Pando, C., Urschitz, J., Moisyadi, S., Schnuetgen, F., Kawasaki, H., Holt, M. G.. 2018-11-28. Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy. https://doi.org/10.1101/481580

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