Hi-C yields chromosome-length scaffolds for a legume genome, Trifolium subterraneum

Dudchenko, O.; Pham, M.; Lui, C.; Batra, S. S.; Hoeger, M.; Nyquist, S. K.; Durand, N. C.; Shamim, M. S.; Machol, I.; Erskine, W.; Aiden, E. L.; Kaur, P.

doi:10.1101/473553

bioRxiv · 10.1101/473553

Hi-C yields chromosome-length scaffolds for a legume genome, Trifolium subterraneum

Dudchenko, O.·Pham, M.·Lui, C.·Batra, S. S.·Hoeger, M.·Nyquist, S. K.·Durand, N. C.·Shamim, M. S.·Machol, I.·Erskine, W.·Aiden, E. L.·Kaur, P.

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Abstract

We present a chromosome-length assembly of the genome of subterranean clover, Trifolium subterraneum, a key Australian pasture legume. Specifically, in situ Hi-C data (48X) was used to correct misjoins and anchor, order, and orient scaffolds in a previously published genome assembly (TSUd_r1.1; scaffold N50: 287kb). This resulted in an improved genome assembly (TrSub3; scaffold N50: 56Mb) containing eight chromosome-length scaffolds that span 95% of the sequenced bases in the input assembly.

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Dudchenko, O., Pham, M., Lui, C., Batra, S. S., Hoeger, M., Nyquist, S. K., Durand, N. C., Shamim, M. S., Machol, I., Erskine, W., Aiden, E. L., Kaur, P.. 2018-11-20. Hi-C yields chromosome-length scaffolds for a legume genome, Trifolium subterraneum. https://doi.org/10.1101/473553

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