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bioRxiv · 10.1101/472589

Leveraging collective regulatory effects of long-range DNA methylations to predict gene expressions and estimate their effects on phenotypes in cancer

Kim, S.·Park, H. J.·Cui, X.·Zhi, D.
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Abstract

DNA methylation of various genomic regions plays an important role in regulating gene expression in diverse biological contexts. However, most genome-wide studies have focused on the effect of 1) methylation in cis, not in trans and 2) a single CpG, not the collective effects of multiple CpGs, on gene expression. In this study, we developed a statistical machine learning model, geneEXPLORER (gene expression prediction by long-range epigenetic regulation), that quantifies the collective effects of both cis- and trans- methylations on gene expression. By applying geneEXPLORER to The Cancer Genome Atlas (TCGA) breast and lung cancer data, we found that most genes are affected by methylations of as much as 10Mb from promoter regions or more, and the long-range methylation explains 50% of the variation in gene expression on average, far greater than cis-methylation. The highly predictive genes are related to breast cancer, especially oncogenes and suppressor genes. Further, the predicted gene expressions could predict clinical phenotypes such as breast tumor status and estrogen receptor status (AUC=0.999, 0.94 respectively) as accurately as the measured gene expression levels. These results suggest that geneEXPLORER provides a means for accurate imputation of gene expression, which can be further used to predict clinical phenotypes.

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Kim, S., Park, H. J., Cui, X., Zhi, D.. 2018-11-19. Leveraging collective regulatory effects of long-range DNA methylations to predict gene expressions and estimate their effects on phenotypes in cancer. https://doi.org/10.1101/472589

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