Remote control of alternative splicing in roots through TOR kinase

Petrillo, E.; Riegler, S.; Fuchs, A.; Servi, L.; Godoy Herz, M. A.; Kubaczka, M. G.; Venhuizen, P.; Schweighofer, A.; Kornblihtt, A. R.; Simpson, C.; Brown, J. W. S.; Meyer, C.; Kalyna, M.; Barta, A.

doi:10.1101/472126

bioRxiv · 10.1101/472126

Remote control of alternative splicing in roots through TOR kinase

Petrillo, E.·Riegler, S.·Fuchs, A.·Servi, L.·Godoy Herz, M. A.·Kubaczka, M. G.·Venhuizen, P.·Schweighofer, A.·Kornblihtt, A. R.·Simpson, C.·Brown, J. W. S.·Meyer, C.·Kalyna, M.·Barta, A.

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Abstract

For plants, light is the source of energy and the most relevant regulator of growth and adaptations to the environment by inducing changes in gene expression at various levels, including alternative splicing. Chloroplasts trigger retrograde signals that control alternative splicing in leaves and roots in response to light. Here we provide evidence suggesting that sugars, derived from photosynthesis, act as mobile signals controlling alternative splicing in roots. The inhibition of TOR kinase activity diminishes the alternative splicing response to light and/or sugars in roots, showing the relevance of the TOR pathway in this signaling mechanism. Furthermore, disrupting the function of the mitochondria abolishes alternative splicing changes supporting a key role for these organelles in this signaling axis. We conclude that sugars can act as mobile signals coordinating alternative splicing responses to light throughout the whole plant, exerting this function in roots by activating the TOR pathway.\n\nGraphical Abstract\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=200 SRC=\"FIGDIR/small/472126v2_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (76K):\norg.highwire.dtl.DTLVardef@a8c978org.highwire.dtl.DTLVardef@1430da0org.highwire.dtl.DTLVardef@1339990org.highwire.dtl.DTLVardef@a6dd5a_HPS_FORMAT_FIGEXP M_FIG C_FIG Art by Dr. Luciana Giono.

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Petrillo, E., Riegler, S., Fuchs, A., Servi, L., Godoy Herz, M. A., Kubaczka, M. G., Venhuizen, P., Schweighofer, A., Kornblihtt, A. R., Simpson, C., Brown, J. W. S., Meyer, C., Kalyna, M., Barta, A.. 2018-11-19. Remote control of alternative splicing in roots through TOR kinase. https://doi.org/10.1101/472126

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