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bioRxiv · 10.1101/465039

High-throughput micro-patterning platform reveals Nodal-dependent dissection of peri-gastrulation-associated versus pre-neurulation associated fate patterning

Tewary, M.·Dziedzicka, D.·Ostblom, J.·Prochazka, L.·Shakiba, N.·Woodford, C.·Piccinini, E.·Vickers, A.·Louis, B.·Rahman, N.·Danovi, D.·Geens, M.·Watt, F. M.·Zandstra, P. W.
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Abstract

In vitro models of post-implantation human development are valuable to the fields of regenerative medicine and developmental biology. Here, we report characterization of a robust in vitro platform that enabled high-content screening of multiple human pluripotent stem cell (hPSC) lines for their ability to undergo peri-gastrulation-like fate patterning upon BMP4 treatment of geometrically-confined colonies and observed significant heterogeneity in their differentiation propensities along a gastrulation associable and neuralization associable axis. This cell line associated heterogeneity was found to be attributable to endogenous nodal expression, with upregulation of Nodal correlated with expression of a gastrulation-associated gene profile, and Nodal downregulation correlated with a neurulation-associated gene profile expression. We harness this knowledge to establish a platform of pre-neurulation-like fate patterning in geometrically confined hPSC colonies that arises due to a stepwise activation of reaction-diffusion and positional-information. Our work identifies a Nodal signalling dependent switch in peri-gastrulation versus pre-neurulation-associated fate patterning in hPSC cells, provides a technology to robustly assay hPSC differentiation outcomes, and suggests conserved mechanisms of self-organized fate specification in differentiating epiblast and ectodermal tissues.

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Tewary, M., Dziedzicka, D., Ostblom, J., Prochazka, L., Shakiba, N., Woodford, C., Piccinini, E., Vickers, A., Louis, B., Rahman, N., Danovi, D., Geens, M., Watt, F. M., Zandstra, P. W.. 2018-11-07. High-throughput micro-patterning platform reveals Nodal-dependent dissection of peri-gastrulation-associated versus pre-neurulation associated fate patterning. https://doi.org/10.1101/465039

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