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bioRxiv · 10.1101/464545

Data-Independent Acquisition Mass Spectrometry to Localize Phosphosites

Li, Q.-R.·Wang, S.-S.·Zhu, H.-W.·Xia, F.-Y.·Roepstorff, P.·Wu, J.-R.·Zeng, R.
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Abstract

In conventional data-dependent acquisition (DDA) mode used for phosphoproteome analysis by mass spectrometry, a considerable number of phosphosites are localized ambiguously. We found that data-independent acquisition (DIA) method could localize phosphosite accurately and confidently due to its acquisition nature of repeated and unbiased mode. Here, we present a robust and promising DIA workflow for modification sites determination. Through dedicated collection and analyses of site-determining ions following their chromatographic profilings, phosphosites could be assigned or rectified unambiguously.

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Li, Q.-R., Wang, S.-S., Zhu, H.-W., Xia, F.-Y., Roepstorff, P., Wu, J.-R., Zeng, R.. 2018-11-07. Data-Independent Acquisition Mass Spectrometry to Localize Phosphosites. https://doi.org/10.1101/464545

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