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bioRxiv · 10.1101/457069

High-throughput determination of the antigen specificities of T cell receptors in single cells

Zhang, S.-Q.·Ma, K.-Y.·Schonnesen, A. A.·Zhang, M.·He, C.·Sun, E.·Williams, C. M.·Jia, W.·Jiang, N.
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Abstract

We present tetramer-associated T-cell receptor sequencing (TetTCR-Seq), a method to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-Seq to identify patterns in TCR cross-reactivity with cancer neo-antigens and to rapidly isolate neo-antigen-specific TCRs with no cross-reactivity to the wild-type antigen.

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Zhang, S.-Q., Ma, K.-Y., Schonnesen, A. A., Zhang, M., He, C., Sun, E., Williams, C. M., Jia, W., Jiang, N.. 2018-11-01. High-throughput determination of the antigen specificities of T cell receptors in single cells. https://doi.org/10.1101/457069

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