Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli

Patino-Navarrete, R.; Rosinski-Chupin, I.; Cabanel, N.; Gauthier, L.; Takissian, J.; Madec, J.-Y.; Hamze, M.; Bonnin, R. A.; Naas, T.; Glaser, P.

doi:10.1101/446195

bioRxiv · 10.1101/446195

Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli

Patino-Navarrete, R.·Rosinski-Chupin, I.·Cabanel, N.·Gauthier, L.·Takissian, J.·Madec, J.-Y.·Hamze, M.·Bonnin, R. A.·Naas, T.·Glaser, P.

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Abstract

Carbapenem-resistant Enterobacteriaceae are considered by WHO as \"critical\" priority pathogens for which novel antibiotics are urgently needed. The dissemination of carbapenemase-producing Escherichia coli (CP-Ec) in the community is a major public health concern. However, the global molecular epidemiology of CP-Ec isolates, as well as the genetic bases for the emergence and global dissemination of specific lineages, remain largely unknown. Here, by combining a thorough genomic and evolutionary analysis of Ec ST410 isolates with a broad analysis of 12,584 E. coli and Shigella genomes, we showed that the fixation of carbapenemase genes depends largely on a combination of mutations in ftsI encoding the penicillin binding protein 3 and in the porin genes ompC and ompF. Mutated ftsI genes and a specific ompC allele inducing reduced susceptibility to diverse {beta}-lactams spread across the species by recombination. The selection of CP-Ec lineages able to disseminate is more complex than the mere acquisition of carbapenemase genes.

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Patino-Navarrete, R., Rosinski-Chupin, I., Cabanel, N., Gauthier, L., Takissian, J., Madec, J.-Y., Hamze, M., Bonnin, R. A., Naas, T., Glaser, P.. 2018-10-17. Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli. https://doi.org/10.1101/446195

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