Fast, volumetric live-cell imaging using high-resolution light-field microscopy

Li, H.; Guo, C.; Kim-Holzapfel, D.; Li, W.; Altshuller, Y.; Schroeder, B.; Liu, W.; Meng, Y.; French, J.; Takamaru, K.-I.; Frohman, M.; Jia, S.

doi:10.1101/439315

bioRxiv · 10.1101/439315

Fast, volumetric live-cell imaging using high-resolution light-field microscopy

Li, H.·Guo, C.·Kim-Holzapfel, D.·Li, W.·Altshuller, Y.·Schroeder, B.·Liu, W.·Meng, Y.·French, J.·Takamaru, K.-I.·Frohman, M.·Jia, S.

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Abstract

Visualizing diverse anatomical and functional traits that span many spatial scales with high spatio-temporal resolution provides insights into the fundamentals of living organisms. Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric functional brain imaging. Given those promising applications at the tissue level, at its other extreme, this highly-scalable approach holds great potential for observing structures and dynamics in single-cell specimens. However, the challenge remains for current LFM to achieve subcellular level, near-diffraction-limited 3D spatial resolution. Here, we report high-resolution LFM (HR-LFM) for live-cell imaging with a resolution of 300-700 nm in all three dimensions, an imaging depth of several micrometers, and a volume acquisition time of milliseconds. We demonstrate the technique by imaging various cellular dynamics and structures and tracking single particles. The method may advance LFM as a particularly useful tool for understanding biological systems at multiple spatio-temporal levels.

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Li, H., Guo, C., Kim-Holzapfel, D., Li, W., Altshuller, Y., Schroeder, B., Liu, W., Meng, Y., French, J., Takamaru, K.-I., Frohman, M., Jia, S.. 2018-10-10. Fast, volumetric live-cell imaging using high-resolution light-field microscopy. https://doi.org/10.1101/439315

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