Accurate registration of 3D time-lapse microscopy images

Mousavi Kahaki, S. M.; Wang, S.-L.; Stepanyants, A.

doi:10.1101/436824

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bioRxiv · 10.1101/436824

Accurate registration of 3D time-lapse microscopy images

Mousavi Kahaki, S. M.·Wang, S.-L.·Stepanyants, A.

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Abstract

In vivo imaging experiments often require automated detection and tracking of changes in the specimen. This problem, however, can be hindered by variations in the position and orientation of the specimen relative to the microscope, as well as by linear and nonlinear deformations. Here, we present a feature-based registration method, coupled with translation, rigid, affine, and B-spline transformations, designed to address these issues in 3D time-lapse microscopy images. In this method, features are detected as local intensity maxima in the source and target image stacks, and their similarity matrix is used as an input to the Hungarian algorithm to establish initial correspondences. Random Sampling Consensus algorithm is then employed to eliminate outliers. The resulting set of corresponding features is used to determine the optimal transformations. Accuracy of the proposed algorithm was tested on fluorescently labeled axons imaged over a 48-day period with a two-photon laser scanning microscope. For this, multiple axons in individual stacks of images were traced semi-manually in 3D, and the distances between the corresponding traces were measured before and after the registration. The results show that there is a progressive improvement in the registration accuracy with increasing complexity of the transformations. In particular, an accuracy of less than 1 voxel (0.26 m) was achieved with a regularized B-spline transformation. To illustrate the utility of the proposed method, registered images were used to automatically track synaptic boutons on axons over the entire duration of the experiment, yielding 99% precision and recall.

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Mousavi Kahaki, S. M., Wang, S.-L., Stepanyants, A.. 2018-10-05. Accurate registration of 3D time-lapse microscopy images. https://doi.org/10.1101/436824

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