Molecular detection of virulence and resistance genes in Salmonella enterica serovar Typhi and Paratyphi A, B and C isolated from human diarrhea samples and lettuce in Burkina Faso.

Aly, S.; Somda, N. S.; Bonkoungou, J. I. O.; Traore, O.; Sambe-Ba, B.; Wane, A. A.; Traore, Y.; Gassama-Sow, A.

doi:10.1101/436501

Home/Archive/bioRxiv

bioRxiv · 10.1101/436501

Molecular detection of virulence and resistance genes in Salmonella enterica serovar Typhi and Paratyphi A, B and C isolated from human diarrhea samples and lettuce in Burkina Faso.

Aly, S.·Somda, N. S.·Bonkoungou, J. I. O.·Traore, O.·Sambe-Ba, B.·Wane, A. A.·Traore, Y.·Gassama-Sow, A.

View original source ↗Export metadata ↓

Abstract

ObjectivesIn Burkina Faso (BF), dirty water, in particular those of the stoppings and the gutter are used for irrigation of vegetables. The aim of this study is to contribute to the knowledge on the molecular level of Salmonella Typhi and Paratyphi circulating in the hospitals and environment next to hospitals in BF.\n\nMethodsSalmonella Typhi and Paratyphi strains isolated from patients between 2009 to 2015 and lettuce samples isolated in 2014 in BF were characterized by simple PCR using specific primers.\n\nResultsOut of 100 Salmonella isolated, 53% were from human and 47% from lettuce samples. Globally, the highest prevalence was observed with invA, misL, pipD, orfL and spvR genes in 97%, 96%; 74%; and 21%. Forty of these isolates carried class 1 integron, 31 from clinical samples and 9 from lettuce samples. Sequencing showed seven different gene cassette arrangements, with aadA1 in 13/15 strains, aadA7 and aac(3)-Id in 2/15 strains. Eight percent (8/100) of Salmonella harbored gyrB and parE genes with 6 from clinical and 2 from lettuce isolates. Sequencing showed no mutation in these genes. Three distinct PFGE types were observed from clinical samples with 90-95% similarity in each case. All Salmonella from lettuce had similar pulsotypes.\n\nConclusionThis study showed the diversity virulence and resistance genes harbored of S. Typhi and Paratyphi from both clinical and lettuce samples in BF. Lettuce is a potential source of transmission of Salmonella causing diarrhea among human in BF.

Explore related subjects

microbiology Preprint

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Aly, S., Somda, N. S., Bonkoungou, J. I. O., Traore, O., Sambe-Ba, B., Wane, A. A., Traore, Y., Gassama-Sow, A.. 2018-10-05. Molecular detection of virulence and resistance genes in Salmonella enterica serovar Typhi and Paratyphi A, B and C isolated from human diarrhea samples and lettuce in Burkina Faso.. https://doi.org/10.1101/436501

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Tandem repeats in methanogenic archaea and their viruses reveal a shared potential for rapid genomic diversification

Methanogenic archaea drive global methane emissions, yet the molecular basis of their physiological flexibility is unclear. Archaeal adaptation is typically attributed to horizontal gene transfer, which is episodic and cannot explain rapid, reversible change. Here we show that direct tandem repeats (TRs) offer an intrinsic alternative. By screening 50,567 prokaryotic genomes, we find TR densities in Methanosarcina more than 30-fold above the prokaryotic average, paralleling Borg extrachromosomal elements. These TRs recur at conserved loci while sharing almost no sequence identity between strains, indicating independent origin, and evolve during routine cultivation. Methanogen TRs are transcribed, show ribosome occupancy, and lie near regulatory and cofactor-related genes, whilst being depleted around core energy-conserving and translational machinery. Genic TRs generate intrinsically disordered regions in proteins carrying nucleotide-binding, protein-protein interaction and cell-surface domains, and occur in structural and surface proteins of methanogen-infecting viruses. TRs are therefore an overlooked source of variability and adaptation potential in methanogens and their viruses.

microbiology↗

bioRxiv2026

DarHTG is a tripartite phage defence system for purine DNA base ADP-ribosylation

Bacteria evolved a variety of phage defence strategies to combat the constant onslaught of viral threats. DarTG toxin-antitoxin systems provide phage defence by ADP-ribosylation of bases in single-stranded DNA which interferes with viral replication and aborts phage infection. Here, we describe DarHTG, a system widespread among bacteria, that combines DarTG with a non-processive superfamily I helicase functioning as co-toxin for DarT and thereby enabling the ADP-ribosylation of double-stranded DNA. We identified two such DarHTG subclasses which catalyse the ADP-ribosylation of adenosine and guanosine bases, respectively, and reverse these modifications with structurally unique ARH superfamily antitoxins. Thus, DarHTG antitoxins expand the known macromolecular target spectrum of the ARH enzyme class to nucleic acids. These DarHTG systems confer resistance to a broad range of bacteriophages and can be triggered by the highly charged tails of viral single-stranded DNA-binding proteins, which occlude the active site of the ARH antitoxin.

microbiology↗

bioRxiv2026

Draft genome of Paraperlucidibaca sp. CMO1 isolated from marine oil snow in the Churchill Marine Observatory, MB, Canada

An oil-degrading enrichment culture was inoculated from Marine Oil Snow (MOS) collected during an oil-spill experiment at the Churchill Marine Observatory in winter 2024. The complete metagenome-assembled genome for Paraperlucidibaca sp. CMO1 was reconstructed, exhibiting the genetic potential to degrade hydrocarbons, including alkanes and aromatic compounds, as well as the ability to produce surfactants.

microbiology↗