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bioRxiv · 10.1101/422865

Streptococcus salivarius - a potential salivary biomarker for orofacial granulomatosis and Crohn’s disease?

Goel, R. M.·Prosdocimi, E. M.·Amar, A.·Omar, Y.·Escudier, M. P.·Sanderson, J. D.·Wade, W. G.·Prescott, N.
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Abstract

ObjectiveOrofacial granulomatosis (OFG) is a rare disease characterised by chronic, non-caseating, granulomatous inflammation primarily affecting the oral cavity. Histologically, it is similar to Crohns disease (CD) and a proportion of patients have both OFG and CD. The cause of OFG remains elusive but it has been suggested that microbial interactions may be involved. The aim of this study was to compare the salivary microbial composition of subjects with OFG and/or CD and healthy controls.\n\nDesign261 subjects were recruited, of whom 78 had OFG only, 40 had both OFG and CD, 97 had CD only with no oral symptoms and 46 were healthy controls. Bacterial community profiles were obtained by sequencing the V1-V3 region of the 16S rRNA gene.\n\nResultsThere were no differences in richness or diversity of the salivary bacterial communities between patient groups and controls. The relative abundance of the Streptococcus salivarius-group were raised in patients with OFG or CD only compared to controls while that of the Streptococcus mitis -group was lower in CD compared to both OFG and controls. One S. salivarius oligotype made the major contribution to the increased proportions seen in patients with OFG and CD.\n\nConclusionThe salivary microbiome of individuals with OFG and CD was similar to that found in health although the proportions of S. salivarius, a common oral Streptococcus were raised. One specific strain-level oligotype was found to be primarily responsible for the increased levels seen.

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Goel, R. M., Prosdocimi, E. M., Amar, A., Omar, Y., Escudier, M. P., Sanderson, J. D., Wade, W. G., Prescott, N.. 2018-09-23. Streptococcus salivarius - a potential salivary biomarker for orofacial granulomatosis and Crohn’s disease?. https://doi.org/10.1101/422865

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