High-throughput identification of dominant negative polypeptides in yeast

Dorrity, M. W.; Queitsch, C.; Fields, S.

doi:10.1101/418608

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bioRxiv · 10.1101/418608

High-throughput identification of dominant negative polypeptides in yeast

Dorrity, M. W.·Queitsch, C.·Fields, S.

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Abstract

Dominant negative polypeptides can act as inhibitors by binding to the wild type protein or by titrating an essential ligand. Here, we use high-throughput sequencing of DNA libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth. The method uncovers numerous inhibitory polypeptides for a protein and thus is capable of defining interacting domains with exquisite resolution, even pinpointing individual residues that contact ligands.

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Dorrity, M. W., Queitsch, C., Fields, S.. 2018-09-15. High-throughput identification of dominant negative polypeptides in yeast. https://doi.org/10.1101/418608

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