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bioRxiv · 10.1101/417899

Modeling the Electro-chemical Properties of Microbial Opsin ChrimsonR for Application to Optogenetics-based Vision Restoration

Sabatier, Q.·Joffrois, C.·Gauvais, G.·Chavas, J.·Pruneau, D.·Picaud, S.·Benosman, R.
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Abstract

Optogenetic activation of neurons [1] have greatly contributed to our understanding of how neural circuits operate, and holds huge promise in the field of neural prosthetics, particularly in sensory restoration. The discovery of new channelrhodopsins, Chrimson -- which is 45 nm more red-shifted than any previously discovered or engineered channelrhodopsin -- and its mutant ChrimsonR with faster kinetics [2] made this technology available for medical applications. However, a detailed model that would be able to accurately reproduce the membrane potential dynamics in cells transfected with ChrimsonR under light stimulation is missing. We address this issue by developing the first model for the electrochemical behavior of ChrimsonR that predicts its conductance in response to arbitrary light stimulation. Our model captures ON and OFF dynamics of the protein for stimuli with frequencies up to 100 Hz and their relationship with the brightness, as well as its activation curve, the steady-state amplitude of the response as a function of light intensity. Additionally, we capture a slow adaptation mechanism at a timescale at the order of minutes. Our model holds for light intensities covering the whole dynamic range of the channel (from response onset to saturation) and for timescales in the order of up to several minutes. This model is a new step towards modeling the spiking activity of ChrimsonR-expressing neurons, required for the precise control of information transmission in optogenetics-based Brain-Computer Interfaces, and will inform future applications of ChrimsonR based optogenetics.

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Sabatier, Q., Joffrois, C., Gauvais, G., Chavas, J., Pruneau, D., Picaud, S., Benosman, R.. 2018-09-14. Modeling the Electro-chemical Properties of Microbial Opsin ChrimsonR for Application to Optogenetics-based Vision Restoration. https://doi.org/10.1101/417899

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