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bioRxiv · 10.1101/415455

CLAMP directly interacts with MSL2 to facilitate Drosophila dosage compensation

Tikhonova, E.·Fedotova, A.·Bonchuk, A.·Mogila, V.·Larschan, E. N.·Georgiev, P. G.·Maksimenko, O.
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Abstract

The binding of Drosophila male-specific lethal (MSL) dosage compensation complex exclusively to male X chromosome provides an excellent model system to understand mechanisms of selective recruitment of protein complexes to chromatin. Previous studies showed that the male-specific organizer of the complex, MSL2, and ubiquitous DNA-binding protein CLAMP are key players in the specificity of X chromosome binding. The CXC domain of MSL2 binds to genomic sites of MSL complex recruitment. Here we demonstrated that MSL2 directly interacts with the N-terminal zinc-finger domain of CLAMP. CLAMP-MSL2 and CXC-DNA interactions are cooperatively involved in recruitment of MSL complex to the X chromosome.

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Tikhonova, E., Fedotova, A., Bonchuk, A., Mogila, V., Larschan, E. N., Georgiev, P. G., Maksimenko, O.. 2018-09-12. CLAMP directly interacts with MSL2 to facilitate Drosophila dosage compensation. https://doi.org/10.1101/415455

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