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bioRxiv · 10.1101/413088

Cell type-specific complement expression from healthy and diseased retinae

Pauly, D.·Schäfer, N.·Grassmann, F.·Pfaller, A. M.·Straub, T.·Weber, B. H.·Hauck, S. M.·Grosche, A.
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Abstract

Retinal degeneration is associated with complement system activation, but retinal sources of complement are unknown. Here, we describe the human and murine complement transcriptomes of Muller cells, microglia/macrophages, vascular cells, neurons and retinal pigment epithelium (RPE) in health and disease. All cell populations expressed c1s, c3, cfb, cfp, cfh and cfi. Murine Muller cells contributed the highest amount of complement activators (c1s, c3, cfb). RPE mainly expressed cfh, while cfi and cfp transcripts were most abundant in neurons. The main complement negative regulator in the human retina was cfi, while cfh dominated in the murine retina. Importantly, the expression of c1s, cfb, cfp, cfi increased and that of cfh decreased with aging. Impaired photoreceptor recycling led to an enhanced c3 expression in RPE and to a reduced cfi expression in microglia/macrophages. Expression of complement components was massively upregulated after transient retinal ischemia in murine microglia, Muller cells and RPE. The individual signature of complement expression in distinct murine and human retinal cell types indicates a local, well-orchestrated regulation of the complement system in both species.

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Pauly, D., Schäfer, N., Grassmann, F., Pfaller, A. M., Straub, T., Weber, B. H., Hauck, S. M., Grosche, A.. 2018-09-10. Cell type-specific complement expression from healthy and diseased retinae. https://doi.org/10.1101/413088

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