Molecular safeguarding of CRISPR gene drive experiments

Champer, J.; Chung, J.; Lee, Y. L.; Liu, C.; Yang, E.; Wen, Z.; Clark, A. G.; Messer, P. W.

doi:10.1101/411876

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bioRxiv · 10.1101/411876

Molecular safeguarding of CRISPR gene drive experiments

Champer, J.·Chung, J.·Lee, Y. L.·Liu, C.·Yang, E.·Wen, Z.·Clark, A. G.·Messer, P. W.

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Abstract

CRISPR-based gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate the suitability of synthetic target sites and split drives as flexible safeguarding strategies for gene drive experiments.

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Champer, J., Chung, J., Lee, Y. L., Liu, C., Yang, E., Wen, Z., Clark, A. G., Messer, P. W.. 2018-09-08. Molecular safeguarding of CRISPR gene drive experiments. https://doi.org/10.1101/411876

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