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bioRxiv · 10.1101/407908

Integrin αVβ6-EGFR crosstalk regulates bidirectional force transmission and controls breast cancer invasion

Thomas, J. R.·Moore, K. M.·Sproat, C.·Maldonado, H.·Mo, S.·Haider, S.·Hammond, D. E.·Thomas, G. J.·Prior, I. A.·Cutillas, P. R.·Jones, L. J.·Marshall, J. F.·Morgan, M. R.
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Abstract

The mechanical properties of the extracellular matrix within tumours control multiple cellular functions that drive cancer invasion and metastasis. However, the mechanisms controlling microenvironmental force sensation and transmission, and how these regulate transcriptional reprogramming and invasion, are unclear. Our aim was to understand how mechanical inputs are transmitted bidirectionally and translated into biochemical and transcriptional outputs to drive breast cancer progression. We reveal that adhesion receptor and growth factor receptor crosstalk regulates a bidirectional feedback mechanism co-ordinating force-dependent transcriptional regulation and invasion.\n\nIntegrin V{beta}6 drives invasion in a range of carcinomas and is a potential therapeutic target. V{beta}6 exhibits unique biophysical properties that promote force-generation and increase matrix rigidity. We employed an inter-disciplinary approach incorporating proteomics, biophysical techniques and multi-modal live-cell imaging to dissect the role of V{beta}6-EGFR crosstalk on transmission of mechanical signals bidirectionally between the extracellular matrix and nucleus.\n\nWe show that V{beta}6 expression correlates with poor prognosis in triple-negative breast cancer (TNBC) and drives invasion of TNBC cells. Moreover, our data show that a complex regulatory mechanism exists involving crosstalk between V{beta}6 integrin and EGFR that impacts matrix stiffness, force transmission to the nucleus, transcriptional reprogramming and microenvironment rigidity. V{beta}6 engagement triggers EGFR & MAPK signalling and V{beta}6-EGFR crosstalk regulates mutual receptor trafficking mechanisms. Consequently, EGF stimulation suppresses V{beta}6-mediated force-application on the matrix and nuclear shuttling of force-dependent transcriptional co-activators YAP/TAZ. Finally, we show that crosstalk between V{beta}6 & EGFR regulates TNBC invasion.\n\nWe propose a model whereby V{beta}6-EGFR crosstalk regulates matrix stiffening, but also the transmission of extracellular forces into the cell in order to co-ordinate transcriptional reprogramming and invasion. To exploit adhesion receptors and receptor tyrosine kinases therapeutically, it will be essential to understand the integration of their signalling functions and how crosstalk mechanisms influence invasion and the response of tumours to molecular therapeutics.

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Thomas, J. R., Moore, K. M., Sproat, C., Maldonado, H., Mo, S., Haider, S., Hammond, D. E., Thomas, G. J., Prior, I. A., Cutillas, P. R., Jones, L. J., Marshall, J. F., Morgan, M. R.. 2018-09-04. Integrin αVβ6-EGFR crosstalk regulates bidirectional force transmission and controls breast cancer invasion. https://doi.org/10.1101/407908

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