Genome-scale Capture C promoter interaction analysis implicates novel effector genes at GWAS loci for bone mineral density

Chesi, A.; Wagley, Y.; Johnson, M. E.; Manduchi, E.; Su, C.; Lu, S.; Leonard, M. E.; Hodge, K. M.; Pippin, J. A.; Hankenson, K. D.; Wells, A. D.; Grant, S. F. A.

doi:10.1101/405142

bioRxiv · 10.1101/405142

Genome-scale Capture C promoter interaction analysis implicates novel effector genes at GWAS loci for bone mineral density

Chesi, A.·Wagley, Y.·Johnson, M. E.·Manduchi, E.·Su, C.·Lu, S.·Leonard, M. E.·Hodge, K. M.·Pippin, J. A.·Hankenson, K. D.·Wells, A. D.·Grant, S. F. A.

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Abstract

ASBTRACTOsteoporosis is a devastating disease with an essential genetic component. Genome wide association studies (GWAS) have discovered genetic variants robustly associated with bone mineral density (BMD), however they only report genomic signals and not necessarily the precise localization of culprit effector genes. Therefore, we sought to carry out physical and direct variant to gene mapping in a relevant primary human cell type. We developed SPATIaL-seq (genome-Scale, Promoter-focused Analysis of chromaTIn Looping), a massively parallel, high resolution Capture-C based method to simultaneously characterize the genome-wide interactions of all human promoters. By intersecting our SPATIaL-seq and ATAC-seq data from human mesenchymal progenitor cell -derived osteoblasts, we observed consistent contacts between candidate causal variants and putative target gene promoters in open chromatin for ~30% of the 110 BMD loci investigated. Knockdown of two novel implicated genes, ING3 at CPED1-WNT16 and EPDR1 at STARD3NL, had pronounced inhibitory effects on osteoblastogenesis. Our approach therefore aids target discovery in osteoporosis and can be applied to other common genetic diseases.

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Chesi, A., Wagley, Y., Johnson, M. E., Manduchi, E., Su, C., Lu, S., Leonard, M. E., Hodge, K. M., Pippin, J. A., Hankenson, K. D., Wells, A. D., Grant, S. F. A.. 2018-08-31. Genome-scale Capture C promoter interaction analysis implicates novel effector genes at GWAS loci for bone mineral density. https://doi.org/10.1101/405142

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