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bioRxiv · 10.1101/394981

Centromeric CENP-A loading requires accurate mitotic timing, which is linked to checkpoint proteins

Erhardt, S.·Pauleau, A.-L.·Bergner, A.·Kajtez, J.
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Abstract

A defining feature of centromeres is the presence of the histone H3 variant CENP-A that replaces H3 in a subset of centromeric nucleosomes. In Drosophila cultured cells CENP-A deposition at centromeres takes place during the metaphase stage of the cell cycle and strictly depends on the presence of its specific chaperone CAL1. How CENP-A loading is restricted to mitosis is unknown. We found that CAL1 interacts with the spindle assembly checkpoint protein and RZZ complex component Zw10 and thus constitutes the anchor for the recruitment of RZZ. We identify Zw10 as determinant of a mitotic duration and show that it is inversely correlated with CENP-A levels at centromeres. Moreover, overexpression of CAL1 is associated with increased CENP-A levels at centromeres and completely uncouples CENP-A loading from mitosis. Therefore CAL1 controls CENP-A incorporation at centromeres both quantitatively and temporally, connecting it to the spindle assembly checkpoint to further ensure mitotic fidelity.

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Erhardt, S., Pauleau, A.-L., Bergner, A., Kajtez, J.. 2018-08-17. Centromeric CENP-A loading requires accurate mitotic timing, which is linked to checkpoint proteins. https://doi.org/10.1101/394981

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