Preliminary Study of the Potential Mechanism of CTSD in the Aging Process of Sepiella japonica: Fundamental Function Analysis

Guo, B.; Chen, Y.; Qi, P.; Ye, Y.; Lv, Z.; Zhang, X.; Xu, K.; Yu Chen, Pengzhi Qi, Yingying Ye, Zhenming Lv, Xiumei Zhang, Kaida Xu,

doi:10.1101/392688

bioRxiv · 10.1101/392688

Preliminary Study of the Potential Mechanism of CTSD in the Aging Process of Sepiella japonica: Fundamental Function Analysis

Guo, B.·Chen, Y.·Qi, P.·Ye, Y.·Lv, Z.·Zhang, X.·Xu, K.·Yu Chen, Pengzhi Qi, Yingying Ye, Zhenming Lv, Xiumei Zhang, Kaida Xu,

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Abstract

Cathepsin D, a kind of endopeptidase, can degrade peptides and proteins in lysosomes, which are involved in cell apoptosis. Previous transcriptome analysis of optic glands of Sepiella japonica across four growth stages, expression of a cathepsin D-like segment was found to be significantly different. Based on the complete cDNA sequence of S. japonica, the CTSD gene (also called sjCTSD, GenBank accession no. KY745896.1) was cloned using RACE amplification; this gene is 1389 bp in length and encodes proteins composed of 393 amino acids. Spatio-temporal expression profiles of the sjCTSD gene were determined using qPCR assays, which showed that the expression levels of sjCTSD constantly increased across four growth stages in 9 of 11 tissues that were investigated. In the optic glands, as well as pancreas and liver cells, sjCTSD expression levels sharply increased during the post-spawning phase. To investigate the potential role of the sjCTSD gene in aging progress, we constructed the prokaryotic expression vector of pET28a/sjCTSD. After induced by IPTG, the recombinant protein sjCTSD was obtained in the form of inclusion bodies, with a molecular size of approximately 40.3 kDa, the inclusion body of sjCTSD can be converted to soluble protein through the denaturation and renaturation. The results of functional experiments showed that sjCTSD could degrade bovine hemoglobin under acidic conditions, and inhibit the growth of Escherichia coli and Vibrio alginolyticus, which was speculated that the increased expression of sjCTSD may help inhibit the invasion of pathogenic bacteria with the immune function of cuttlefish declines during the aging process. To a certain extent, these results indicated the potential functional role of the sjCTSD gene in the aging process of S. japonica. This study provides insights to further understand the roles of lysosomal proteins on anti-aging effects in S. japonica and other cephalopoda species.

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Guo, B., Chen, Y., Qi, P., Ye, Y., Lv, Z., Zhang, X., Xu, K., Yu Chen, Pengzhi Qi, Yingying Ye, Zhenming Lv, Xiumei Zhang, Kaida Xu,. 2018-08-15. Preliminary Study of the Potential Mechanism of CTSD in the Aging Process of Sepiella japonica: Fundamental Function Analysis. https://doi.org/10.1101/392688

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