Analysis and comparison of genome editing using CRISPResso2

Clement, K.; Rees, H.; Canver, M.; Gehrke, J.; Farouni, R.; Hsu, J.; Cole, M.; Liu, D. R.; Joung, J. K.; Bauer, D. E.; Pinello, L.

doi:10.1101/392217

bioRxiv · 10.1101/392217

Analysis and comparison of genome editing using CRISPResso2

Clement, K.·Rees, H.·Canver, M.·Gehrke, J.·Farouni, R.·Hsu, J.·Cole, M.·Liu, D. R.·Joung, J. K.·Bauer, D. E.·Pinello, L.

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Abstract

Genome editing technologies are rapidly evolving, and analysis of deep sequencing data from target or off-target regions is necessary for measuring editing efficiency and evaluating safety. However, no software exists to analyze base editors, perform allele-specific quantification or that incorporates biologically-informed and scalable alignment approaches. Here, we present CRISPResso2 to fill this gap and illustrate its functionality by experimentally measuring and analyzing the editing properties of six genome editing agents.

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Clement, K., Rees, H., Canver, M., Gehrke, J., Farouni, R., Hsu, J., Cole, M., Liu, D. R., Joung, J. K., Bauer, D. E., Pinello, L.. 2018-08-15. Analysis and comparison of genome editing using CRISPResso2. https://doi.org/10.1101/392217

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