droplet-Tn-Seq combines microfluidics with Tn-Seq identifying complex single-cell phenotypes

Thibault, D.; Wood, S.; Jensen, P.; van Opijnen, T.

doi:10.1101/391045

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bioRxiv · 10.1101/391045

droplet-Tn-Seq combines microfluidics with Tn-Seq identifying complex single-cell phenotypes

Thibault, D.·Wood, S.·Jensen, P.·van Opijnen, T.

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Abstract

While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. droplet-Tn-seq solves that problem by microfluidics facilitated encapsulation of individual transposon mutants into liquid-in-oil droplets, thereby enabling isolated growth, free from the influence of the population. Importantly, all advantages of Tn-Seq are conserved, while reducing costs and greatly extending its applicability.

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Thibault, D., Wood, S., Jensen, P., van Opijnen, T.. 2018-08-13. droplet-Tn-Seq combines microfluidics with Tn-Seq identifying complex single-cell phenotypes. https://doi.org/10.1101/391045

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