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bioRxiv · 10.1101/389502

Software tools for automated transmission electron microscopy

Schorb, M.·Haberbosch, I.·Hagen, W.·Schwab, Y.·Mastronarde, D.
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Abstract

In the recent years, electron microscopy in the life sciences has witnessed increasing demand for high-throughput data collection in both structural and cellular biology. We present a combination of software tools that enable automated acquisition guided by image analysis for a wide variety of Transmission Electron Microscopy applications. Using these tools, we demonstrate dose-reduction in single particle cryo-EM experiments, fully automated acquisition of every single cell in a plastic section and automated targeting of features on serial sections for 3D volume imaging even across multiple grids.

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Schorb, M., Haberbosch, I., Hagen, W., Schwab, Y., Mastronarde, D.. 2018-08-10. Software tools for automated transmission electron microscopy. https://doi.org/10.1101/389502

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