Imperfect Linkage Disequilibrium Generates Phantom Epistasis (& Perils of Big Data)

Abstract

The genetic architecture of complex human traits and diseases is affected by large number of possibly interacting genes, but detecting epistatic interactions can be challenging. In the last decade, several studies have alluded to problems that linkage disequilibrium can create when testing for epistatic interactions between DNA markers. However, these problems have not been formalized nor have their consequences been quantified in a precise manner. Here we use a conceptually simple three locus model involving a causal locus and two markers to show that imperfect LD can generate the illusion of epistasis, even when the underlying genetic architecture is purely additive. We describe necessary conditions for such \"phantom epistasis\" to emerge and quantify its relevance using simulations. Our empirical results demonstrate that phantom epistasis can be a very serious problem in GWAS studies (with rejection rates against the additive model greater than 0.2 for nominal p-values of 0.05, even when the model is purely additive). Some studies have sought to avoid this problem by only testing interactions between SNPs with R-sq. <0.1. We show that this threshold is not appropriate and demonstrate that the magnitude of the problem is even greater with large sample size. We conclude that caution must be exercised when interpreting GWAS results derived from very large data sets showing strong evidence in support of epistatic interactions between markers.