Pervasive Transcription Fine-tunes Replication Origin Activity

Candelli, T.; Gros, J.; Libri, D.

doi:10.1101/384859

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bioRxiv · 10.1101/384859

Pervasive Transcription Fine-tunes Replication Origin Activity

Candelli, T.·Gros, J.·Libri, D.

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Abstract

RNA polymerase (RNAPII) transcription occurs pervasively, which raises the important question of its functional impact on other DNA-associated processes, including replication. In budding yeast, replication originates from Autonomously Replicating Sequences (ARSs), generally located in intergenic regions. The influence of transcription on ARSs function has been studied for decades, but these earlier studies have necessarily neglected the role of non-annotated transcription. We studied the relationships between pervasive transcription and replication origin activity using high-resolution transcription maps. We show that ARSs alter the pervasive transcription landscape by pausing and terminating neighboring RNAPII transcription, thus limiting the occurrence of pervasive transcription within origins. We provide evidence that quasi-symmetrical binding of the ORC complex to ARS borders is responsible for pausing/termination. We also show that low, physiological levels of pervasive transcription impact the function of replication origins. Overall, our results have important implications for understanding the impact of genomic location on origin function.

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Candelli, T., Gros, J., Libri, D.. 2018-08-04. Pervasive Transcription Fine-tunes Replication Origin Activity. https://doi.org/10.1101/384859

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